findkm

Function

Description

findkm reads a file of enzymatic reaction data (substrate concentration [S] versus reaction velocity [V]) and draws Michaelis Menten and Hanes Woolf plots of the data. From these it calculates the Michaelis Menten constant (Km) and the maximum velocity (Vmax) of the reaction. An output file with all relevant data is written.

Usage

Command line arguments

Input file format

The input file is a file of enzymatic data, [S] against V, Substrate concentration against reaction velocity.

Output file format

Data files

None.

Notes

None.

References

  1. Voet & Voet (1993) 'Biochemistry'
  2. Dawes 'Quantitative Problems in Biochemistry' 5th Edition.
  3. Manuel G. Claros, Francisco M. Canovas 'Lines&Kinetics: a graphic tool to deal with linear regressions and enzyme kinetics.' EMBnet.news vol 5.1 http://www.uk.embnet.org/embnet.news/vol5_1/kinetics.html
  4. Cornish-Bowden (1996) 'Fundamentals of Enzyme kinetics' Portland Press, London.

Warnings

None.

Diagnostic Error Messages

None.

Exit status

It exits with a status of 0.

Known bugs

None.

Author(s)

This application was modified by

History

Target users

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